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Fig. 5 | Stem Cell Research & Therapy

Fig. 5

From: EDTA soluble chemical components and the conditioned medium from mobilized dental pulp stem cells contain an inductive microenvironment, promoting cell proliferation, migration, and odontoblastic differentiation

Fig. 5

Effects of the EDTA extracts and the CM on proliferation, migration, cell attachment, odontoblastic differentiation, and endothelial cell differentiation in vitro. a Proliferation effect of the EDTA extracts and the CM analyzed by CCK-8® in C2C12 cells. b Migration effect of the EDTA extracts and the CM analyzed by TAXIScan-FL® in C2C12 cells. c Effect of the EDTA extracts and the CM on cell attachment ability analyzed by Giemza stain in C2C12 cells. d Effect of the EDTA extracts and the CM on odontoblast differentiation analyzed by RT-PCR in C2C12 cells. e Angiogenic effect of the EDTA extracts and the CM by immunocytochemical analysis with vascular endothelial (VE)-cadherin in HUVEC. Data are expressed as means ± SD of four determinations. *P < 0.05, **P < 0.01. CM conditioned medium, DSPP dentin sialophosphoprotein

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Stem Cell Research & Therapy

ISSN: 1757-6512