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Table 1 Custom-designed DNA primers used in real-time quantitative PCR study

From: Angiopoietin-1 receptor Tie2 distinguishes multipotent differentiation capability in bovine coccygeal nucleus pulposus cells

Gene

Forward sequence

Reverse sequence

18S

ACGGACAGGATTGACAGATTG

CCAGAGTCTCGTTCGTTATCG

TEK

GGACAGGCAATAAGGATACG

ACCGAGTGGATGAAGGAA

COL2

CGGGTGAACGTGGAGAGACA

GTCCAGGGTTGCCATTGGAG

ACAN

GGCATCGTGTTCCATTACAG

ACTCGTCCTTGTCTCCATAG

HIF1α

AGGTGGATATGTCTGGATA

CAAGTCGTGCTGAATAATAC

  1. Amplicons were generated using a two-step amplification cycling (95 °C for 15 s and 57 °C for 30 s for 45 cycles) and SYBR-green mastermix
  2. TEK angiopoietin-1 receptor gene, COL2 collagen type II gene, ACAN aggrecan gene, HIF1α hypoxia-inducible factor 1 alpha gene, 18S ribosomal 18S RNA
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Stem Cell Research & Therapy

ISSN: 1757-6512