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Fig. 8 | Stem Cell Research & Therapy

Fig. 8

From: Properties of internalization factors contributing to the uptake of extracellular DNA into tumor-initiating stem cells of mouse Krebs-2 cell line

Fig. 8

Internalization of protamine/extracellular DNA complexes by Krebs-2 cells. a Analysis of GFP fluorescence in Krebs-2 cells following incubation with pEGFP-N1 plasmid. DAPI represents chromatin (blue channel); FITC represents GFP (green channel, no signal). b Transformation of E. coli competent cells with DNA isolated from Krebs-2 cells incubated with pEGFP-N1 or pEGFP-N1/protamine complexes. c FACS analysis of Alu-TAMRA DNA/protamine internalization by Krebs-2 cells: 1: Negative control, intact Krebs-2 cells; 2: Krebs-2 + protamine; 3: Krebs-2 + Alu-TAMRA DNA; 4: Krebs-2 + Alu-TAMRA DNA/protamine complexes. Percentage values correspond to autofluorescence levels observed in the control and to the fraction of TAMRA+ cells at experimental points

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Stem Cell Research & Therapy

ISSN: 1757-6512