Fig. 2
From: Sphere-forming cells from peripheral cornea demonstrate the ability to repopulate the ocular surface
Immunostaining of peripheral corneal spheres stimulated by collagen I substrate reveals expression of cell proliferation and differentiation markers. Spheres were imaged at 20× objective magnification by confocal microscopy and labelled with antibodies (green signal) for keratin 3 a and vimentin b. The secondary-antibody-only control c did not show this signal. Blue signals represent DAPI staining of DNA within cell nuclei. Red signals represent EDU-incorporated cell nuclei indicative of proliferating cells. At 60× magnification, vimentin-positive migrated cells proximal to the sphere d show a different morphology compared with distal cells e and some show positivity for the EDU cell proliferation marker (red) f. Scale bar =100 μm (Colour figure online)