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Fig. 3 | Stem Cell Research & Therapy

Fig. 3

From: Sphere-forming cells from peripheral cornea demonstrate the ability to repopulate the ocular surface

Fig. 3

Implantation of peripheral corneal spheres into donor corneoscleral rims. Spheres (arrowheads) were implanted into wedge-shaped incisions made at the limbal region. Under stereomicroscopy, the corneoscleral rim with incisions (arrows) and implanted spheres can be clearly visualized a. Combined phase-contrast and fluorescence microscopy show an implanted sphere stained positively for live cells with LIVE/DEAD® stain b. This signal is confined to the sphere and not detected in the surrounding tissue. A 40× DAPI-stained 10-μm thick cross-section of frozen-stored corneoscleral rim confirmed the absence of DAPI-positive cell nuclei prior to implantation c. Through phase-contrast microscopy, the position of the spheres in the semi-transparent region of tissue is shown d

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