Fig. 6
From: Sphere-forming cells from peripheral cornea demonstrate the ability to repopulate the ocular surface
Immunocytochemistry of peripheral corneal spheres implanted into the limbal region (dotted region of interest a) of corneoscleral tissue and cultured for 14 days. Montage imaging showing monolayer of vimentin-stained cells (green) having migrated over the ‘anterior surface’ of the corneal bed a. Confocal imaging of immunostained cross-sections at 60× objective magnification shows ∆Np63α-positive staining (green) surrounding a cell nucleus a1 (arrowhead), and laminin-positive staining a2 showing clusters of strong green signals in tissue not associated with cells (arrows) and weaker positive signals close to or within the cell (arrowheads). Vimentin-positive green signals are seen associated with cell nuclei a3 and a representative image of the secondary-antibody-only negative control shows no non-specific staining a4. Whole-mount sections show a positive signal for ABCG2 b and notch 1 c. Representative image of the secondary-antibody-only negative control d shows no green positive-staining. Blue signals represent DAPI staining of DNA within cell nuclei. Scale bar = 100 μm for a1–a4, 50 μm for a and 100 μm for b, d (Colour figure online)