Fig. 7
From: Sphere-forming cells from peripheral cornea demonstrate the ability to repopulate the ocular surface
Expression of keratocan, laminin A1 and PCNA in corneal peripheral spheres implanted into corneoscleral tissue and cultured over 4, 7 and 14 days. All data are calibrated to non-implanted spheres (dotted line). Expression data (normalized to β-actin and GAPDH) are expressed as percentages of non-implanted spheres ± 1 standard error of spheres collected from three separate donors. Keratocan and laminin expression declined significantly post implantation. PCNA, however, was significantly elevated 4 days post implantation. There was a significant decrease in PCNA expression from day 4 to day 7. By days 7 and 14, PCNA expression is statistically equivalent to non-implanted sphere PCNA expression. Black square for day 4 implant indicates data available from only two donor sets due to tissue processing limitations. *p < 0.05. PCNA proliferating cell nuclear antigen