Table 1 Quantitative PCR primers used in this study
From: Sphere-forming cells from peripheral cornea demonstrate the ability to repopulate the ocular surface
Product of interest | Primer sets | Size of cDNA amplicon (base pairs) | Size of gDNA amplicon (base pairs) |
|---|---|---|---|
ATP-binding cassette, sub-family G (WHITE), member 2 (ABCG2) | (F): CCTGAGATCCTGAGCCTTTG (R): AAGCCATTGGTGTTTCCTTG | 124 | 184,966 |
Proliferating cell nuclear antigen (PCNA) | (F): GGCGTGAACCTCACCAGTAT (R): TTCTCCTGGTTTGGTGCTTC | 125 | 0 |
Vimentin (VIM) | (F): CCAAACTTTTCCTCCCTGAACC (R): GTGATGCTGAGAAGTTTCGTTGA | 141 | 1395 |
Laminin, alpha 1 (LAMA1) | (F): ACACCGGGAAGTGTCTGAAC (R): GCTTGAGGAGCACCTTTCAC | 239 | 0 |
Keratocan (KERA) | (F): ATCTGCAGCACCTTCACCTT (R): CATTGGAATTGGTGGTTTGA | 167 | 4043 |
Actin beta (β-actin) | (F): AACTCCATCATGAAGTGTGACG (R): GATCCACATCTGCTGGAAG | 234 | 345 |
Glycreraldehyde-3-phosphate dehydrogenase (GAPDH) | (F): CTGACTTCAACAGCGACACC (R): CCCTGTTGCTGTAGCCAAAT | 120 | 224 |
ABCB5 | (F): TACTCTTCCCACTGCCATTG (R): CAATTATCCATCAAGACCATCTATCAAG (Probe): 56-FAM/CCGACCAAG/ZEN/GCGACTGTCTCT/3IABkFQ | 106 | 0 |
p63alpha | (F): GGGTCGTGAAATAGTCCAGAC (R): CATCCACCTCCCACTGC (Probe): 56-FAM/CACCTCCGT/ZEN/ATCCCACAGATTGCA/3IABkFQ | 108 | 0 |
alphaSMA | (F): CTGTTGTAGGTGGTTTCATGGA (R): AGAGTTACGAGTTGCCTGATG (Probe): 56-FAM/AGACCCTGT/ZEN/TCCAGCCATCCTTC/3IABkFQ | 131 | 0 |
Notch1 | (F): ACAGATGCCCAGTGAAGC (R): CGAGGTCAACACAGACGAG | 112 | 1289 |