Fig. 5From: Single-stranded DNA binding protein Ssbp3 induces differentiation of mouse embryonic stem cells into trophoblast-like cellsOverexpression of Ssbp3 decreases the methylation level at the Elf5 promoter in mouse ESCs. Clonal bisulfite sequencing of the 1 kb promoter region of Elf5 in ESCs, TSCs, and ESCs overexpressing Ssbp3 were shown. Each line represents an individual clone with open circles depicting unmethylated sites and filled circles indicating methylated sites. The CpG dinucleotide at –355 bp is polymorphic and absent where shaded gray. ES C embryonic stem cell, TSC trophoblast stem cellBack to article page