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Fig. 7 | Stem Cell Research & Therapy

Fig. 7

From: Single-stranded DNA binding protein Ssbp3 induces differentiation of mouse embryonic stem cells into trophoblast-like cells

Fig. 7

Teratomas derived from Ssbp3-overexpressing ESCs contain hemorrhage. a Gross appearance of teratomas derived from control cells or Ssbp3-overexpressing ESCs (upper panel). The number of teratomas examined is presented in the table (lower panel). b The net weight of teratomas derived from control cells and Ssbp3-overexpressing ESCs. c Cross-section of teratomas derived from control cells (upper panel) or Ssbp3-overexpressing ESCs (lower panel). d Histology of teratomas derived from control cells (upper panel) or Ssbp3-overexpressing ESCs (lower panel) showing tissue complexity (hematoxylin and eosin staining). Arrowheads mark the endoderm (black), mesoderm (blue), and ectoderm (green) cells. e Hematoxylin and eosin-stained images for sections of a teratoma derived from Ssbp3-overexpressing ESCs. The trophoblast cluster (arrow heads, left panel) and trophoblast giant cells with the enlarged nuclei (arrow heads, right panel) are indicated. f qRT-PCR analysis for expression levels of trophoblast-specific markers in teratomas derived from control cells or Ssbp3-overexpressing ESCs. The average mRNA level in teratomas derived from control cells was set at 1.0. Data are shown as mean ± SD (n = 3). *p < 0.05, **p < 0.01. OE overexpression

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