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Fig. 3 | Stem Cell Research & Therapy

Fig. 3

From: Multipotency and cardiomyogenic potential of human adipose-derived stem cells from epicardium, pericardium, and omentum

Fig. 3

ADSC and BJ fibroblast (BJF) ALP activity (ac) and constitutive ALP gene expression (d, e). Comparison of ALP activity quantitated by colorimetric analysis of the constitutive enzyme activity in passage 5 ADSCs and BJ fibroblasts (n = 5, mean ± SEM, *P < 0.05 vs E-ADSCs) (a). ALP staining in the same donor epicardial (b) and pericardial (c) ADSCs. Quantitative real-time RT-PCR results performed in duplicate represented by fold-change values as compared with fibroblasts, with a standard 18S endogenous control. Comparison of the collected ADSC subtype samples (n = 5 adipose tissue samples for each ADSC subtype, mean ± SEM, *P < 0.05 vs E-ADSCs) (d), and epicardium and pericardium-derived stem cells harvested from the same brain-dead organ donor free of comorbidities (e). ALP alkaline phosphatase, E/O/P-ADSC epicardial/omental/pericardial adipose-derived stem cell

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