Fig. 3From: Multipotency and cardiomyogenic potential of human adipose-derived stem cells from epicardium, pericardium, and omentumADSC and BJ fibroblast (BJF) ALP activity (a–c) and constitutive ALP gene expression (d, e). Comparison of ALP activity quantitated by colorimetric analysis of the constitutive enzyme activity in passage 5 ADSCs and BJ fibroblasts (n = 5, mean ± SEM, *P < 0.05 vs E-ADSCs) (a). ALP staining in the same donor epicardial (b) and pericardial (c) ADSCs. Quantitative real-time RT-PCR results performed in duplicate represented by fold-change values as compared with fibroblasts, with a standard 18S endogenous control. Comparison of the collected ADSC subtype samples (n = 5 adipose tissue samples for each ADSC subtype, mean ± SEM, *P < 0.05 vs E-ADSCs) (d), and epicardium and pericardium-derived stem cells harvested from the same brain-dead organ donor free of comorbidities (e). ALP alkaline phosphatase, E/O/P-ADSC epicardial/omental/pericardial adipose-derived stem cellBack to article page