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Fig. 3 | Stem Cell Research & Therapy

Fig. 3

From: Local injection of bone marrow progenitor cells for the treatment of anal sphincter injury: in-vitro expanded versus minimally-manipulated cells

Fig. 3

Area of sphincterotomy and repair (SR) at 30 days after operation. Cryostat sections at low magnification (left column) and same field at higher magnification (right column). Toluidine blue staining. CTR (a, b), MSC (c, d), MNC (e, f), and sham operated (g, h) animals. At day 30 the lesion is still visible in CTR animals (a, b) as a large gap in the muscular layer (M) filled with dense connective tissue and mast cells. It is instead almost completely repaired and recognizable as a limited gap in the muscle layer in the animals that received intralesion injection of stem cells either in-vitro expanded (c) or minimally manipulated (e). At higher magnification (d, f), numerous small clusters of smooth muscle cells are irregularly interspersed in the fibrous connective tissue. In sham operated animals the muscular layer appeared intact (g, h). Magnification: a, c, e, g × 2; b, d, f, h same field × 4

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