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Fig. 1 | Stem Cell Research & Therapy

Fig. 1

From: Comparison of a xeno-free and serum-free culture system for human embryonic stem cells with conventional culture systems

Fig. 1

Morphology of hESC colonies cultured in different conditions. a hESC-1 showed a differentiated state after five passages cultured in XF-HFF/CDM conditions. b hESC-1 maintained an undifferentiated state cultured in XF-HFF/CDM conditions after 40 passages. c Morphology of hESC-1 cultured in XF-HFF/HS medium after five passages is elongated with a high nucleus/cytoplasm ratio, but a low propagation rate. d Morphology of hESC-1 cultured on HS-matrix in CDM medium after five passages is elongated with a low nucleus/cytoplasm ratio and propagation rate. e Morphology of hESC-1 cells on XC-HFF in KSR medium after 45 passages was still undifferentiated with a high nucleus/cytoplasm ratio and high propagation rate. Scale bar represents 50 μm

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