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Fig. 2 | Stem Cell Research & Therapy

Fig. 2

From: Comparison of a xeno-free and serum-free culture system for human embryonic stem cells with conventional culture systems

Fig. 2

Expression of stem cell markers in hESC-1 grown on XF-HFF in CDM conditions after >30 passages. a Immunofluorescence staining with antibodies of stem cell markers: SSEA3 (upper left), SSEA4 (upper left), TRA-1-60 (lower left), and TRA-1-81 (lower right). b RT-PCR analysis of the expression of stem cell markers OCT3/4, hTERT, SOX2, and Nanog, with GAPDH as positive control. Scale bar represents 50 μm. GAPDH glyceraldehyde-3-phosphate dehydrogenase, hTERT human telomerase reverse transcriptase, OCT octamer-binding transcription factor, SSEA stage-specific embryonic antigen, Tra tumor rejection antigen

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