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Fig. 5 | Stem Cell Research & Therapy

Fig. 5

From: Comparison of a xeno-free and serum-free culture system for human embryonic stem cells with conventional culture systems

Fig. 5

Comparison of different hESC culture systems. a Growth curve of hESCs cultured in four different conditions: 4 × 104 cells from each culture condition were plated on day 0. Cell numbers were counted from triplicate wells at days 3, 5, and 7 after transferring from the initial XC-HFF/KSR condition. b Cells cultured in different conditions were analyzed on day 6 by fluorescence-activated cell sorting for SSEA-4 and TRA-1-60. n = 3. Error bars indicate SD. CDM chemically-defined medium, HS human serum, KSR KnockOut Serum Replacement, SR serum replacement, SSEA stage-specific embryonic antigen, Tra tumor rejection antigen, XF-HFF xeno-free human foreskin fibroblast feeders

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