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Fig. 6 | Stem Cell Research & Therapy

Fig. 6

From: Comparison of a xeno-free and serum-free culture system for human embryonic stem cells with conventional culture systems

Fig. 6

Expression levels of hESC markers under different culture systems. a Concentrations of bFGF in different hESC culture media were collected after overnight incubation at 4 or 37 °C. b Quantitative RT-PCR TaqMan analysis of Oct-4 and hTERT expression in hESCs maintained in various conditions. Samples were measured by ELISA for final FGF2 concentrations. Values with SDs are listed below bars in nanograms per milliliter. n = 3. *p < 0.05, **p < 0.01. Error bars indicate SD. bFGF basic fibroblast growth factor, CDM chemically-defined medium, HS human serum, hTERT human telomerase reverse transcriptase, KSR KnockOut Serum Replacement, OCT octamer-binding transcription factor, XF-HFF xeno-free human foreskin fibroblast feeders

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Stem Cell Research & Therapy

ISSN: 1757-6512