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Fig. 2 | Stem Cell Research & Therapy

Fig. 2

From: Combined administration of mesenchymal stem cells overexpressing IGF-1 and HGF enhances neovascularization but moderately improves cardiac regeneration in a porcine model

Fig. 2

Insulin-like growth factor-1 (IGF-1) and hepatocyte growth factor (HGF) overexpression in pig mesenchymal stem cells from adipose tissue (paMSC) after lentiviral transduction. a α-IGF-1 immunocytochemistry in paMSC transduced with the pRRLsin18.CMV-IGF-1-IRES-GFP lentiviral vector (positive fractions). Negative controls (–) show evaluation of Cherry + cells; scale bars = 20 μm. b α-HGF immunocytochemistry in paMSC transduced with the pRRLsin18.CMV-HGF-IRES-Cherry lentiviral vector (positive fractions); analysis was performed after cell separation. Negative controls (–) show evaluation of green fluorescent protein (GFP) + cells; nuclei were DAPI-stained; scale bars = 20 μm. c Relative expression of selected markers and genes to characterize paMSC-mod cells. IGF-1, IGF-1R, GFP, and Cherry expression relative to the endogenous GusB gene in the cell populations (n = 2; *p ≤ 0.05). d Western blot analysis of HGF in several cell populations transduced with the pRRLsin-18.CMV-HGF-IRES-Cherry vector. HEK293T cell lines were used as a control; α-tubulin was used as a loading control

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