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Fig. 2 | Stem Cell Research & Therapy

Fig. 2

From: Characterization of intercellular communication and mitochondrial donation by mesenchymal stromal cells derived from the human lung

Fig. 2

BM-MSCs, LT-MSCs and BAL-MSCs donate cytoplasmic contents to BEAS2B epithelial cells. Cytoplasmic transfer between mesenchymal stromal cells (MSCs) and BEAS2B bronchial epithelial cells was measured by flow cytometry and immunofluorescence. a Representative flow cytometry plot of transfer after MSCs were labelled with Calcein AM and co-cultured with BEAS2B cells for 4 hours. Blue line represents co-cultured BEAS2B cells, red line represents unstained control. b To assess whether epithelial cells were capable of donating cytoplasmic material to MSCs, BEAS2B cells were labelled with Calcein AM and co-cultured with MSCs for 4 h. Blue line represents co-cultured MSCs, red line represents unstained control. c Representative flow cytometry plot of transfer after MSCs were labelled with Calcein AM and co-cultured with BEAS2B cells within a transwell configuration.  d Grouped data of cytoplasmic transfer by bone marrow-derived (BM-MSCs), lung tissue-derived (LT-MSCs) and bronchoalveolar fluid-derived (BAL-MSCs) MSCs. Y axis represents the fold-change increase in Calcein AM mean fluorescent intensity (MFI) of BEAS2B cells after co-culture with each respective MSC population. Horizontal bars represent median. eg Representative immunofluorescent images of cytoplasmic transfer by BM-MSCs (e), LT-MSCs (f) and BAL-MSCs (g). MSCs were pre-labelled with Calcein AM (green) and co-cultured with BEAS2B epithelial cells labelled with DiD (red). BEAS2B cells containing MSC-derived Calcein AM appear yellow/orange. Images shown at 630× magnification. Scale bars represent 10 μM

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