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Fig. 2 | Stem Cell Research & Therapy

Fig. 2

From: Adipose tissue-derived stem cells suppress hypertrophic scar fibrosis via the p38/MAPK signaling pathway

Fig. 2

The effect of ADSC-CM on the primary HSFs cultured in vitro. ac qRT-PCR analysis for the mRNA levels of Col1, Col3, and α-SMA in HSFs stimulated for 24 h with different concentrations of ADSC-CM (10 %, 20 %, 40 %, and 80 % concentrations of diluted conditioned medium); the graph represents the expression of Col1, Col3, and α-SMA relative to that of GAPDH. dg Immunoblot analysis for the protein expression of Col1, Col3, and α-SMA in HSFs stimulated for 24 h with different concentrations of ADSC-CM (10 %, 20 %, 40 % and 80 % concentrations of diluted conditioned medium); the graph shows the relative band density to β-actin. Every experiment was repeated at least three times. The data are shown as mean ± SEM (*P < 0.05; **P < 0.01). h Immunofluorescence staining of α-SMA expression in HSFs stimulated with 80 % concentration of ADSC-CM for 24 h. Scale bars = 50 μm. ADSC-CM adipose tissue-derived stem cell-conditioned medium, Col1 collagen I, Col3 collagen III, DAPI 4, 6-diamidino-2-phenylindole, DMEM Dulbecco’s modified Eagle medium, SMA smooth muscle actin

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