Fig. 6

ADSC-CM decreased the expression of collagen and improved the structure and arrangement of collagen through the p38/MAPK signaling pathway in cultured hypertrophic scars tissues ex vivo. a, b Immunoblot analysis for the expression of p-p38 stimulated with DMEM or 80 % concentration of ADSC-CM for 7 days. c The protein levels of p-p38, Col1, Col3, and α-SMA in hypertrophic scar tissues cultured with DMEM or 80 % concentration of ADSC-CM including anisomycin. d–h To verify the role of the p38/MAPK signaling pathway underlying the anti-fibrosis effect of ADSC-CM, SB203580 and/or anisomycin were added into the medium of DMEM cultured scar tissues. The protein expression of Col1, Col3, and α-SMA are shown. Graphs show the relative band density to β-actin. i–m Western blotting analysis for Col1, Col3, and α-SMA in HS tissues cultured with 80 % concentration of ADSC-CM in the presence of p38 inhibitor and/or activator. Graphs show the relative band density to β-actin. Quantitative analysis is shown by histogram. n The structure and arrangement of collagen fibers by Masson’s trichrome staining in DMEM and 80 % ADSC-CM cultured HS tissues in the presence of p38 inhibitor and/or activator. Scale bars = 50 μm. *P < 0.05; **P < 0.01. A anisomycin, ADSC-CM adipose tissue-derived stem cell-conditioned medium, Col1 collagen I, Col3 collagen III, DMEM Dulbecco’s modified Eagle medium, S SB203580, SMA smooth muscle actin