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Fig. 1 | Stem Cell Research & Therapy

Fig. 1

From: High-efficiency generation of induced pluripotent mesenchymal stem cells from human dermal fibroblasts using recombinant proteins

Fig. 1

Characterization of recombinant Yamanaka transcription factors for transduction. a Recombinant refolded and purified Oct4, Sox2, c-Myc, and Klf4 were analyzed using denaturing Bis-Tris NuPage gel. Marker protein standards are shown in lane 1. b Schematic protocol depicting the process and timeline for generating iPMSCs. c Protein transduction and visualization in human fibroblasts 24, 48, 72, and 96 h post HVJ-E transduction of cells. Cells were stained with Hochest 33342 dye for nuclei (blue), and immunostained with anti-His antibody (green) for localization of the recombinant Yamanaka transcription factors. Merged images are shown for 24, 48, 72 and 96 h post transduction. d day, IPSC induced pluripotent stem cell

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Stem Cell Research & Therapy

ISSN: 1757-6512