Skip to main content
Fig. 2 | Stem Cell Research & Therapy

Fig. 2

From: High-efficiency generation of induced pluripotent mesenchymal stem cells from human dermal fibroblasts using recombinant proteins

Fig. 2

Generation of iPMSCs by HVJ-E transduced purified recombinant transcription factors. a ALP staining of putative iPMSCs. Control fibroblasts were without reprogramming and stained with ALP. ALP1 denotes expanded cells with ALP staining. The expanded iPMSCs grew in compact colonies and stained positive for ALP (ALP2). b Putative clone expressing hESC markers (green), including Sox2, Oct4, Nanog, Lin28, Tra1-60, Tra1-81,SSEA-3, and CD24. Nuclei were stained with Hochest 33342 stain (blue) and the images were merged. Scale bars = 50 nM. c FACS analysis of the colonies differentiated into fibroblast-shaped cells demonstrating expression of pluripotent stem cell markers including CD105, CD24, and Sox2 (left). Surface antigen profiling of the whole population of protein-reprogrammed fibroblasts for Nanog, Oct4, and SSEA-3 is also shown (right). d Endogenous pluripotency markers of RNA expression in the reprogrammed fibroblasts determined by qPCR. GAPDH was used for normalization. Note that total RNA was extracted from a mixed population of cells with ~30 % displaying stem cell characteristics. *p < 0.01 between control fibroblasts before and after reprogramming using the protein factors. IPMSC induced pluripotent mesenchymal stem cell

Back to article page