Table 1 Primers for quantitative PCR reactions
Gene name | Forward primer | Reverse primer |
|---|---|---|
β-actin | CATGTACGTTGCTATCCAGGC | CTCCTTAATGTCACGCACGAT |
Klf4 | CCCACATGAAGCGACTTCCC | CAGGTCCAGGAGATCGTTGAA |
Nanog | TTTGTGGGCCTGAAGAAAACT | AGGGCTGTCCTGAATAAGCAG |
c-Myc | GGCTCCTGGCAAAAGGTCA | CTGCGTAGTTGTGCTGATGT |
Sox2 | GCCGAGTGGAAACTTTTGTCG | GGCAGCGTGTACTTATCCTTCT |
Oct4 | CTGGGTTGATCCTCGGACCT | CCATCGGAGTTGCTCTCCA |
ABCG2 | CAGGTGGAGGCAAATCTTCGT | ACCCTGTTAATCCGTTCGTTTT |
hTERT | TAATGGGCTCCTTTCACCTG | CAGTGCGTCTTGAGGAGCA |
Insulin | GCAGCCTTTGTGAACCAACAC | CCCCGCACACTAGGTAGAGA |
Pdx1 | ATCTCCCCATACGAAGTGCC | CGTGAGCTTTGGTGGATTTCAT |
Nkx6.1 | GGACTGCCACGCTTTAGCA | TGGGTCTCGTGTGTTTTCTCT |
C/EBPα | CTTCAGCCCGTACCTGGAG | GGAGAGGAAGTCGTGGTGC |
PPARγ | GGGATCAGCTCCGTGGATCT | TGCACTTTGGTACTCTTGAAGTT |