Fig. 2

CD90-negative selection of shRNA CD90 MSCs by magnetic-activated cell sorting. Mesenchymal stromal cells (MSCs) expressing short hairpin (sh)RNA CD90 (shRNA CD90 MSC) were magnetically labelled with CD90 microbeads, and MSC populations negatively selected for CD90 (CD90-negative MSCs). a Histogram superposition: isotype control (shaded histogram grey), non-transduced MSCs (black slim line), shRNA control MSCs (grey line), shRNA CD90 MSCs (blue line), and CD90-negative MSCs (black thick line).Representative histograms from a dental pulp MSC group are shown. b Graph showing mean fluorescence intensity (MFI) of CD90 marker (MFI = MFI marker – MFI isotype) on cells; n = 7, independent experiments with MSCs derived from 7 tissue donors; ****p < 0.001