Fig. 6

Reduction of CD90 expression stimulates MSC osteogenesis. MSCs, shRNA control MSCs, shRNA CD90 MSCs, and CD90-negative MSCs from dental pulp (DPSC), amniotic fluid (AF-MSC) and adipose tissue (ADSC) were tested in parallel for their ability to differentiate in vitro into osteogenic lineages. Calcified deposits were evidenced by Alizarin Red Staining (AR) in cells after 4 weeks of growth in osteogenic induction medium. Calcification was assessed by gross appearance (a) and light microscopy (b). Data shown are representative of multiple replicates. OS osteogenic induction, sh short hairpin