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Fig. 2 | Stem Cell Research & Therapy

Fig. 2

From: Embryonic stem cell preconditioned microenvironment suppresses tumorigenic properties in breast cancer

Fig. 2

Effect of the microenvironment of ES cells on the proliferation of aggressive breast cancer cells. a Microscopy showed a morphological change in cells treated with ES-CM. Scale bar represents 100 μm. b Growth curves made by counting cell numbers every day revealed a decreased proliferation rate of cells treated with ES-CM. *P < 0.05 vs 4T1-CM, ## P < 0.01 vs DMEM, ***P < 0.001vs 4T1-CM, ### P < 0.001 vs DMEM, n = 3. c Trypan blue cell viability assay showed a low survival rate. ***P < 0.001 compared with controls, n = 3. d Fluc imaging showed decreasing bioluminescence signals at 24, 48, and 72 h after culturing cells with ES-CM. e Quantitative analysis of imaging signals. The signal activity showed the suppressed effect growth of ES-CM group. **P < 0.01 vs 4T1-CM, ## P < 0.01 vs DMEM, ***P < 0.001 vs 4T1-CM, ### P < 0.001 vs DMEM, n = 3. CM conditioned medium, DMEM Dulbecco’s modified Eagle medium, ES embryonic stem

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