Skip to main content
Fig. 2 | Stem Cell Research & Therapy

Fig. 2

From: Rapid generation of functional hepatocyte-like cells from human adipose-derived stem cells

Fig. 2

Induction protocols to generate iHeps from human adipose-derived stem cells (hADSCs). a Schematic diagram of hADSC differentiation into iHeps. b The sequential morphological changes from hADSCs to iHeps; I Cell morphology before hepatic induction; II The change of cell morphology with a short spindle shape after 1 day culture in the phase I induction medium; III At day 5, the cell morphology had become polygonal in shape; IV The morphology of hepatocyte-like cells (iHeps). c, d Immunostaining revealed the vast majority of induced cells were positive for the endoderm marker FoxA2 and early hepatocytes marker AFP at the fifth day (upper right: hADSCs). e Gradually increased hepatic gene expression and slowly diminishing or disappearing billiard gene expression during the induction of iHeps. The expression levels of the indicated genes were analyzed by RT-PCR. f Western blot analysis indicated that C18 (45 kDa), AFP (69 kDa), HNF4a (54 kDa), ALB (66 kDa), and AAT (57 kDa) were actively synthesized in hADSC-derived hepatocytes; GAPDH was used as an internal control (35 kDa). Scale bars = 100 µm (b) and 25 µm (c, d). PHH primary human hepatocyte; ATRA All trans retinoic acid

Back to article page