Table 2 Forward (F) and reverse (R) primer pairs used for polymerase chain reactions to detect hepatic specific gene transcripts in human adipose-derived stem cells, induced heaptocytes, and primary human hepatocytes
From: Rapid generation of functional hepatocyte-like cells from human adipose-derived stem cells
Primer | Sequence (5′–3′) |
|---|---|
β-actin | F: TGGACTTCGAGCAAGAGATG |
R: GAAGGAAGGCTGGAAGAGTG | |
CYP1A2 | F: CTTCGCTACCTGCCTAACCC |
R: GACTGTGTCAAATCCTGCTCC | |
CYP1A1 | F: CAAGGGGCGTTGTGTCTTTG |
R: GTCGATAGCACCATCAGGGG | |
CYP2A6 | F: CAGCACTTCCTGAATGAG |
R: AGGTGACTGGGAGGACTTGAGGC | |
CYP2B6 | F: GCACTCCTCACAGGACTCTTG |
R: CCCAGGTGTACCGTGAAGAC | |
CYP2C9 | F: CTACAGATAGGTATTAAGGACA |
R: GCTTCATATCCATGCAGCACCAC | |
CYP2D6 | F: TGAAGGATGAGGCCGTCTGGGAGA |
R: CAGTGGGCACCGAGAAGCTGAAGT | |
CYP3A4 | F: TTCAGCAAGAAGAACAAGGACAA |
R: GGTTGAAGAAGTCCTCCTAAGC |