Fig. 6

Intercellular exchange between endogenous and exogenous MSCs. a Experimental protocol for the in-vivo experiments. Wild-type C57BL/6 N mice received both Dex and Zol administered intravenously. MSCs from GFP-transgenic mice were injected into the mice 1 day after tooth extraction. b A pair of continuous sections was considered the same section. A section was stained with an anti-GFP antibody, while the other was stained with an antibody against CD90 and subjected to mitochondrial staining. As a result, many CD90(+) (total MSCs) and GFP(−) cells (endogenous MSCs) with stained mitochondria were located at the upper portion of the socket (white arrows). Mitochondrial transfer from exogenous MSCs to endogenous MSC was observed in MRONJ model mice. n = 5 for sample, n = 3 for MSC injection. d days, DAPI 4′,6-diamidino-2-phenylindole, GFP green fluorescent protein, i.p. intraperitoneal, i.v. intravenous, MSC mesenchymal stem cell