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Fig. 3 | Stem Cell Research & Therapy

Fig. 3

From: Serum starvation-induced cell cycle synchronization stimulated mouse rDNA transcription reactivation during somatic cell reprogramming into iPSCs

Fig. 3

Western blot analysis of UBF proteins during the iPSC and S-iPSC generation process. a UBF protein expression during the MEF and S-MEF reprogramming process. b Densitometry analysis of UBF proteins during the MEF and S-MEF reprogramming process. Expression level of MEFs was set as 1. c Comparison of UBF protein expression between two types of partially reprogrammed cells. d UBF protein expression of iPSC line (A1), S-iPSC line (S-B3), and R1 ESCs. e Comparison of UBF protein expression between iPSC line (A1), S-iPSC line (S-B3), and R1 ESCs by densitometry analysis. Expression level of R1 ESCs was set as 1. GAPDH was used as an internal reference. The intensities of bands were quantified by densitometry analysis of the Image J analyzer system. Significant differences at *p < 0.05 and **p < 0.01, n = 3. D day, ESC embryonic stem cell, MEF mouse embryonic fibroblast, S-MEF serum-refeeding MEF, UBF upstream binding factor

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Stem Cell Research & Therapy

ISSN: 1757-6512