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Table 1 List of antibodies used for flow cytometry validation

From: Identification and validation of multiple cell surface markers of clinical-grade adipose-derived mesenchymal stromal cells as novel release criteria for good manufacturing practice-compliant production

Antibody

Fluorophore

Company

Catalog number

CD163

FITC

Beckman Coulter

B17492

CD140B

PE

BD

558821

CD248

Alexa Fluor 647a

Abgent

AP6756b

CD146

PC5

Beckman Coulter

A22364

CD200

PE-Cy7

BD

562125

CD36

APC

Beckman Coulter

A87786

CD34

APC-A750

Beckman Coulter

A89309

CD44

Pac Blu

Beckman Coulter

B37789

CD44

PC7

eBioscience

25-0441

CD276

APC

eBioscience

17-2769-41

CD271

FITC

Biolegend

345104

CD105

PE

Beckman Coulter

A07414

CD73

PerCP eFluor710

eBioscience

# 46-0739-42

CD73

PE

BD

#550257

CD90

FITC

Beckman Coulter

IM1839U

CD274

PC7

Beckman Coulter

A78884

CD14

ECD

Beckman Coulter

IM2707U

HLA-ABC

APC

eBioscience

17-9983

HLA-DR

Pac Blu

Beckman Coulter

A74781

CD45

Krome Orange

Beckman Coulter

A96416

  1. APC allophycocyanin, FITC fluorescein isothiocyanate, PC7 R-phycoerythrin cyanin 7, PE R-phycoerythrin
  2. a Custom Beckman Coulter-labeled antibody