Table 1 Primers used in the study
Primer | UniGene | Forward (5′➔3′) | Reverse (5′➔3′) | Amplicon size (base pairs) |
|---|---|---|---|---|
GAPDH | Hs.544577 | GAAGGTGAAGGTCGGAGTC | GAAGATGGTGATGGGATTTC | 225 |
NCAM-All | Hs.503878 | GGACTTCTACCCGGAACATCAG | CGAGCTTAGGTGCACTGGG | Â |
 NCAM-140 (Variant 1), NCAM-125 (Variant 4) |  |  |  | 798 |
 NCAM-180 (Variant 2) |  |  |  | 828 |
 NCAM-120 (Variant 3) |  |  |  | 903 |
 NCAM Variant 5 |  |  |  | 906 |
NCAM-180 | NM_181351.4 | GCTTCGTGGACTCGACCAG | GCAGATGTACTCTCCGGCATC | 126 |
NCAM-140 | NM_000615.6 | CGAAGAAAAGACTCTGGATGG | TCATGCTTTGCTCTCGTTCT | 1498 |
NCAM-125/140 | NM_001242608.1 / NM_000615.6 | CGAAGAAAAGACTCTGGATGG | GTTCCCCTTGGACTGGC | 755 |
NCAM-125 | NM_001242608.1 | CAGCCAGTCCAAGGGG | TGTAGGATGCAGAATTGCCTC | 346 |
NCAM120/125 | NM_001076682.3 / NM_001242608.1 | GAGTATGAGGTCTACGTGGTGGC | GCAGAGAAAAGCAATGAGACAAAG | 152 |
NCAM-120 | NM_001076682.3 | TCTGCTAGCTCGTCTACCCC | CCAAAGGGGGCACTGATCTT | 541 |
NCAM Variant 5 | NM_001242607.1 | GCTTCGTGGACTCGACCAG | GCAGATGTACTCTCCGGCATC | 204 |
ST8SIA2 | Hs.302341 | GGGCAACTCGGGGGTCTTGC | AAGGCCCGCTGGATGACCGA | 162 |
ST8SIA4 | Hs.308628 | ACAGGCGCCGGACACTAAACA | TGCAGCAAACTCCACCACAGGA | 200 |