Skip to main content
Fig. 1 | Stem Cell Research & Therapy

Fig. 1

From: TGFβ-induced switch from adipogenic to osteogenic differentiation of human mesenchymal stem cells: identification of drug targets for prevention of fat cell differentiation

Fig. 1

Effect of TGFβ on adipogenic and osteogenic differentiation of hMSCs. a Triglyceride production by hMSCs, 9 days after incubation with adipogenic differentiation medium in the absence (white shading) and presence (black shading) of 125 ng/ml BMP2 and increasing concentrations of TGFβ1. The enhancing effect of BMP2 is not significant, while the inhibitory effect of TGFβ is significant (p < 0.01) above 1 ng/ml. b Alkaline phosphatase (ALP) activity of hMSCs, 7 days after incubation with osteogenic differentiation medium in the absence (white shading) and presence (black shading) of 125 ng/ml BMP2 and increasing concentrations of TGFβ1. Enhancing effect of BMP2 is significant (p < 0.01) at all data points, while the inhibitory effect of TGFβ is significant (p < 0.01) above 1 ng/ml. c TGFβ-induced switch from adipogenic to osteogenic differentiation. ALP staining (after 7 days) and Oil Red O (ORO) staining (after 9 days) following incubation of hMSCs in adipogenic differentiation medium containing 125 ng/ml BMP2 and the indicated concentrations of TGFβ1. BMP bone morphogenetic protein, TGFβ transforming growth factor beta

Back to article page

Stem Cell Research & Therapy

ISSN: 1757-6512