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Fig. 7 | Stem Cell Research & Therapy

Fig. 7

From: C-reactive protein can upregulate VEGF expression to promote ADSC-induced angiogenesis by activating HIF-1α via CD64/PI3k/Akt and MAPK/ERK signaling pathways

Fig. 7

CD64 mediated CRP-induced VEGF expression regulation in ADSCs. a Immunofluorescence staining of ADSCs for FcγRIII (CD16), FcγRII (CD32), and FcγRI (CD64) expression in ADSCs. b mRNA expression of CD16, CD32, and CD64 detected by PCR amplification and agarose gel electrophoresis. c CRP stimulation significantly increased CD64 mRNA expression in ADSCs, whereas no significant difference was detected in CD16 and CD32, *p < 0.05, versus control. d Effects of CD16, CD16/32, and CD64 blocking antibody on CRP-stimulated (25 μg/ml) VEGF production in ADSCs in which CD64-neutralizing antibody partly abrogated the effect of CRP. e Immunoprecipitation (IP) using antibodies against CRP in ADSCs treated with or without CRP (25 μg/ml). Blotting antibodies were against CRP or FcgRI, *p < 0.05. Columns, mean; bars, SEM. Results are representative of three independent experiments. CRP C-reactive protein, VEGF, vascular endothelial growth factor, HIF-1α hypoxia inducible factor-1α, WB, western blot

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