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Fig. 6 | Stem Cell Research & Therapy

Fig. 6

From: Serial changes in the proliferation and differentiation of adipose-derived stem cells after ionizing radiation

Fig. 6

Osteogenic differentiation. a Bright field view. Calcium deposition (black dots) was scattered throughout the entire area after osteogenic differentiation in all groups. Calcium deposition was condensed and became a mineralized spot (brown and black area) in all groups. b Alkaline phosphatase (AP) activity. All of the groups demonstrated AP activity. Although the 6R group demonstrated the small and round shape morphology of senescent cells, AP activity was also detected in the senescent cells. c Alizarin Red S staining. Mineralization of osteogenically differentiated ASCs was confirmed in all groups by Alizarin Red S staining. d RT-PCR for osteocalcin and type I collagen. No statistically significant differences in osteocalcin type I collagen mRNA levels were detected between the groups

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