Fig. 9

Gel electrophoresis and RT-PCR gene expression of different stem cells. Gel electrophoresis for lipoprotein lipase showed that: lane M (DNA marker) made DNA ladder and lane 4d, 7d, 14d, and 21d showed bands present at suspected size and proved the formation of lipoprotein lipase (arrow) of rat bone marrow mesenchymal stem cells (BMSCs) (a), human adipose tissue-derived mesenchymal stem cells (hADSCs) (b), and human amniotic fluid-derived mesenchymal stem cells (hAFSCs) (c). RT- PCR gene expression showed about a tenfold increase in gene expression of PPAR-γ during adipocytic differentiation (d) and osteocalcin during osteocytic differentiation of MSCs (e) as indicated by the curves above the threshold green line