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Fig. 2 | Stem Cell Research & Therapy

Fig. 2

From: Two-photon polymerized “nichoid” substrates maintain function of pluripotent stem cells when expanded under feeder-free conditions

Fig. 2

Morphology of the embryoid bodies formed by mES cells cultured in the nichoid substrates, compared to flat glass and to kidney ECM. Cells were cultured in the absence of a feeder layer and with LIF up to day 3, then without either a feeder layer or LIF from days 4 to 14. a Phase contrast images at days 3, 7, and 14. The scale bar is 100 μm. b, c SEM images of an embryoid body adhering to the nichoid. d Embryoid body diameter; n = 15, * p < 0.01. The red line shows the dimension of an individual niche. e Number of cells per embryoid body; n = 15, * p < 0.01

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