Fig. 1

Analysis of chemokines in DEB-associated blister fluids and chemokine receptors on the surface of mADSC. a Chemokine profile in blister fluids of Col7a1 ^–/– (DEB) and Col7a1 ^{f lNeo/f lNeo} (Hypo DEB) mice conducted using Proteome Profiler™ Mouse Chemokine Antibody Array. Blister fluids were collected from blister-affected skin (paw, armpit, neck, and abdomen) of newborn mice. Spot intensities were normalized based on background levels and positive control signals. Data shown as mean ± SD from three independent arrays (p > 0.05). b Surface expression of chemokine receptors in primary mADSC on early passage (passages 2–3) detected by flow cytometry. Light gray curves, control antibody signal; dark shaded curves, specific antibody signal. Histograms are representative examples of chemokine receptor expression profiles obtained from five independent mADSC preparations. c Quantitation of flow cytometry results for expression of the chemokine receptors in primary mADSC. Data shown as mean ± SD of cell percentage. DEB dystrophic epidermolysis bullosa