Fig. 2

Analysis of wild-type and DEB-affected skin infiltration with leukocytes. a H&E staining and indirect immunofluorescent analysis of skin infiltration with leukocytes. Detected antigens are shown in respective colors above the panels (CD45, green; CXCR2, green; CD11b, red; colocalization of CD45 or CXCR2 and CD11b, yellow). Nuclei were counterstained with DAPI (blue). bc blister cavity. Scale bar = 100 μm. b Quantitation of the skin-infiltrating cells. Data presented as an average number or percentage of cells (as indicated on the Y axis) per microscopic field ± SD. *p <0.05. **p > 0.05. Data collected from at least 15 independent microscopic fields. CD-positive cell types are shown above the columns. H&E hematoxylin and eosin, WT wild-type skin, NB nonblistering, DEB dystrophic epidermolysis bullosa, Bl blistering DEB-affected skin