Fig. 5

Transplantation of CXCR2-enriched mADSC into DEB-affected mice. a In-vivo live imaging of neonatal DEB mice and 2 and 7 days after IP transplantation of the DiI-labeled native and CXCR2⁺-mADSC (red), respectively. b Direct fluorescent detection of the DiI-mADSC in the blistering skin of the recipient mice at 7 days after transplantation. Blister cavity (bc) outlined by dotted line. c Indirect immunofluorescent detection of type VII and type IV collagens in the skin of mADSC-treated and control mice (indicated above the panels). Detected antigens (in corresponding color) are shown to the left of the panels. Upper row, monochrome images of the sections stained with anti-type VII collagen antibodies. White arrowheads point to the BMZ. Blue, DAPI nuclear staining. Scale bar = 100 μm. DEB dystrophic epidermolysis bullosa, mADSC mouse adipose-derived stem cells