Fig. 1

DPSCs express CD44 on the cell surface. a Live cell images showing signals from anti-CD44 antibody immunofluorescence (green) and Hoechst 33258 nuclear staining (blue). The rightmost panels indicate merged images from double staining. Scale bar: 100 μm. The top panels show DPSCs that were not treated with HA, while the bottom panels show DPSCs treated with HA at 10 μg/mL for 5 min. b Representative single-parameter diagrams showing CD44 expression by flow cytometry. RFI relative fluorescence intensity. c Bar graphs of the FACS analysis data for HA treatment time course of CD44 cell surface expression. Data are presented as the mean ± SD of three different experiments. ^* P < 0.05, vs. control. ^** P < 0.01, vs. control. DPSCs dental pulp stem cells, FITC fluorescein isothiocyanate, HA hyaluronic acid