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Fig. 2 | Stem Cell Research & Therapy

Fig. 2

From: Improved osteogenesis and upregulated immunogenicity in human placenta-derived mesenchymal stem cells primed with osteogenic induction medium

Fig. 2

The osteogenic potential of MSCs and De-MSCs in vitro. a Proliferation of MSCs and De-MSCs detected by CCK-8 during osteogenesis for 7 days (n = 6/group), values of OD presented were mean ± SD, two-tailed unpaired Student t test was applied. b The ALP staining of MSCs and De-MSCs before (0d) and 7d, 14d, and 21d after osteogenic induction. c qRT-PCR analysis of the expression of BMP2, Runx2, Osx (n = 3/group), fold values presented as mean ± SD, one-way ANOVA test was employed for intergroup comparison. * P < 0.05, ** P < 0.01, *** P < 0.001. ALP alkaline phosphatase, BMP2 human bone morphogenetic protein 2, De-MSCs dedifferentiated mesenchymal stem cells, MSCs mesenchymal stem cells, Ob-MSCs MSC-derived osteoblasts, Osx Osterix, Re-MSCs osteoblasts derived from De-MSCs, Runx2 human Runt-related transcription factor 2

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