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Fig. 3 | Stem Cell Research & Therapy

Fig. 3

From: Improved osteogenesis and upregulated immunogenicity in human placenta-derived mesenchymal stem cells primed with osteogenic induction medium

Fig. 3

The osteogenic potential of MSCs and De-MSCs in SCID mice. ad MSCs or De-MSCs cells (1 × 106 cells) were loaded on the collagen scaffolds and implanted subcutaneously in the back of SCID mice. a ALP activity assay of implanted cells 7 days after implantation (n = 3/group), values of P-nitrophenol mmol/L presented were mean ± SD; b qRT-PCR analysis of implanted cells for the expression of BMP2, Runx2, Osx 7 days after implantation (n = 3/group), fold values presented were mean ± SD. c The H&E staining for the implanted scaffolds 30 days after implantation. d Immunohistological staining of collagen II for the implanted scaffolds 30 days after implantation. ** P < 0.01, *** P < 0.001. ALP alkaline phosphatase, BMP2 human bone morphogenetic protein 2, De-MSCs dedifferentiated mesenchymal stem cells, MSCs mesenchymal stem cells, Osx Osterix, Runx2 human Runt-related transcription factor 2

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