Table 1 Primer sequences
Gene | Gene bank accession number | Primer sequence (5′–3′) | Product (base pair) | |
|---|---|---|---|---|
Reference | GAPDH | NM_002046.3 | Forward: GCACCGTCAAGGCTGAGAAC | 138 |
Reverse: TGGTGAAGACGCCAGTGGA | ||||
Osteogenesis | ALP | NM_000478.4 | Forward: CATGCTGAGTGACACAGACAAGAA | 141 |
Reverse: ACAGCAGACTGCGCCTGGTA | ||||
OCN | NM_199173.4 | Forward: ATGAGAGCCCTCAGACTCCTC | 297 | |
Reverse: CGGGCCGTAGAAGCGCCGATA | ||||
Adipogenesis | LPL | NM_000237.2 | Forward: GTCACGGGCTCAGGAGCATTA | 144 |
Reverse: GCTCCAAGGCTGTATCCCAAGA | ||||
PPARγ-2 | NM_015869.4 | Forward: TGGAATTAGATGACAGCGACTTGG | 182 | |
Reverse: CTGGAGCAGCTTGGCAAACA | ||||
Chondrogenesis | ACAN | NM_001135.3 | Forward: ACGAAGACGGCTTCCACCAG | 134 |
Reverse: TCGGATGCCATACGTCCTCA | ||||
COL2A1 | NM_001844.4 | Forward: CCAGTTGGGAGTAATGCAAGGA | 123 | |
Reverse: ACACCAGGTTCACCAGGTTCA |