Fig. 1

Effect of various doses of testicular busulfan treatment. a Changes in body weight of mice injected with various doses of busulfan after 15, 30, 60, and 90 days post BST. Significant (p < 0.0001) reduction in the body weight was observed in IP-BST mice (n = 3). b Macroscopic observation of testis injected with various doses of busulfan at day 15 post BST. Decrease in testis size with increase in dose of busulfan was observed. Remarkable reduction was found in testis size of T-BST-75 and T-BST-100 mice. c Mean testis weight of mice injected with various doses of busulfan. Significant (p < 0.0001) reduction was observed in testis weight of T-BST-75 and T-BST-100 mice (n = 3). d Hematoxylin and eosin-stained sections of testes, injected with different doses of busulfan at the day 15 post BST. (i) No effect was observed in T-BST-25 mice. (ii) Partial evacuation of seminiferous tubule was observed in T-BST-50 mice. (iii) Maximum depletion of germ cells with intact testicular architecture was observed in T-BST-75 mice. (iv) Maximum depletion of germ cells with disturbed testicular architecture was observed in T-BST-100 mice. Scale bar: 100 μm. T-BST-25, T-BST-50, T-BST-75, T-BST-100 denotes mice injected with 25 μg, 50 μg, 75 μg, and 100 μg of busulfan in each testes respectively. T-DMSO denotes control mice injected with DMSO. IP-BST denotes mice injected intraperitoneally with busulfan