Fig. 3

Effect of T-BST-75 on peripheral blood counts and studies of restoration of endogenous spermatogenesis after BST. a–e Graphs showing changes in levels of white blood cells (WBC), red blood cells (RBC), hemoglobin, platelets, and hematocrit of T-BST-75 mice as compared to wild-type control (Wt-Ctrl) mice. No suppression in peripheral blood counts was observed in T-BST-75 mice. f Graph showing caudal epididymal sperm count in T-BST-75 mice 15 days post BST as compared to T-DMSO control mice. No sperm was detected in T-BST-75 mice. (n = 3, p < 0.005). g Hematoxylin and eosin-stained testicular sections of T-BST-75 mice dissected 2 months after BST. Only a few of the non-spermatogenic tubules showed recovery of spermatogenesis at 2 months after BST. Black arrowhead marks the sperm, red arrowhead marks the tubules, in which spermatogenesis has not yet initiated, green arrowhead marks the proliferating Gc. All data were recorded 15 days post BST unless mentioned otherwise. T-BST-75 denotes mice injected with 75 μg of busulfan in each testes, T-DMSO denotes mice injected with DMSO in testes