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Fig. 2 | Stem Cell Research & Therapy

Fig. 2

From: Preclinical study of mouse pluripotent parthenogenetic embryonic stem cell derivatives for the construction of tissue-engineered skin equivalent

Fig. 2

Characteristics of EB differentiation. a EB immunofluorescent staining of undifferentiated state marker SSEA-1 and surface antigens, including cytokeratin (CK), CD73, and CD151. Cell nuclei were stained with DAPI. Bars = 100 μm. b Morphological appearance of pESC-derived and ESC-derived EBs and outgrowths of EBs. Bars = 100 μm. c Expression of Oct3/4 (stemness), nestin (ectoderm), Afp (entoderm), and mesoderm markers Snai1, Hand1, and Gata2 in pESC-derived and ESC-derived EB outgrowths. *p < 0.05, **p < 0.01 vs undifferentiated (UD) cells. Each experiment was repeated three times. EB embryoid body, ESC embryonic stem cell, pESC parthenogenetic embryonic stem cell

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