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Fig. 4 | Stem Cell Research & Therapy

Fig. 4

From: Preclinical study of mouse pluripotent parthenogenetic embryonic stem cell derivatives for the construction of tissue-engineered skin equivalent

Fig. 4

Fibroblastic differentiation of MSCs and growth factor expression evaluation. a Protein expression levels of growth factors in induced and undifferentiated (UD) pESCs and ESCs by ELISA. **p < 0.01 and ## p < 0.01 vs Fs. Each experiment was repeated three times. b Quantitative RT-PCR assay of fibroblast phenotypic hallmarks including Col-I, Col-III, Tn-c, Mmp1, vimentin, and Fsp1 expression 20 days after fibroblastic differentiation. c Morphological appearance of fibroblasts derived from pMSCs and eMSCs after CTGF induction under microscopy analysis. Bars = 100 μm. d Immunofluorescent staining of fibroblasts derived from pMSCs and eMSCs for VIMENTIN, CYTOKERATIN (CK), FSP1, and COL-I. Cell nuclei were stained with DAPI. Bars = 100 μm. eF ESC-derived fibroblast, eMSC MSC derived from ESC, ESC embryonic stem cell, MSC mesenchymal stem cell, pESC parthenogenetic embryonic stem cell, pF pESC-derived fibroblast, pMSC MSC derived from pESC

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