Table 2 Primers of fibroblast phenotypic hallmark genes for quantitative real-time PCR
Gene | Primers (5′–3′) | Product (bp) |
|---|---|---|
Col-I | TCTCCCCCAAGACACAGGAA | 103 |
GCTGGGTAGGGAAGTAGACG | ||
Col-III | ACTGTCCCACGTAAGCACTG | 106 |
CAGGAGGGCCATAGCTGAAC | ||
Tn-c | CCTACTGTCACGCGTCTCTC | 112 |
AAGCCACAACGAGTTCCCAA | ||
Mmp1 | GAGCCACAGATGAGCACAGA | 103 |
AATCTGAACGCTCGCAGTGA | ||
vimentin | CGCTCCTACGATTCACAGCC | 189 |
TGTGGACGTGGTCACATAGC | ||
Fsp1 | CTTCCTGTCCTGCATTGCCA | 112 |
GGCAAACTACACCCCAACAC |