Fig. 1

Flow cytometry analysis of human BMSCs. Human BMSCs (a) between passages 5 to 8 were analyzed by flow cytometry. In all panels, isotype controls are represented by red lines, while immunopositivity for respective cell surface markers are represented by blue lines. Percentages of positive cells as shown within individual panels are representative of one sample. Human BMSCs were found to be highly immunopositive for CD90 (b; 93.90 ± 1.77 %), STRO-1 (c; 93.48 ± 2.48 %), and CD73 (d; 91.76 ± 1.30 %). The neural stem/progenitor marker nestin was also found to be positive in a significant subpopulation of cells (e; 10.31 ± 1.10 %). Hardly any (<1 %) of the cells were immunopositive for the hematopoietic stem cell marker CD45 (f). Mean ± SD, n = 4